Journal: Calcified Tissue International
Article Title: Cxcr2 is Required for Osteoclast Regulation, Bone Structure, and Hematological Response During Bone (Re)modeling
doi: 10.1007/s00223-025-01470-x
Figure Lengend Snippet: Cxcr2 deletion expands the detected pro-inflammatory cytokines and alters systemic bone turnover. a Log-2 transformed fold change in cytokine expression (KO/WT) in Cxcr2 WT versus KO serum, quantified by Eve Technologies 32-Plex Cytokine Array. N = 6 mice per genotype. b Serum concentration of G-CSF and CXCL5 in Cxcr2 WT and KO mice. N = 3–8 mice per genotype. c Serum concentration of osteoblast and osteoclast activity markers P1NP and CTX-1 in Cxcr2 WT and KO mice. N = 4–5 mice per genotype. d Concentration of RANKL and OPG and OPG/RANKL ratio in Cxcr2 WT and KO bone conditioned media. N = 8 samples per group. Error bars represent standard deviation. Analysis of Cxcr2 WT versus KO using Welch’s t- test. Significance levels for differences are indicated: * p < 0.05, ** p < 0.01, *** p < 0.001, ns: not significant ( p ≥ 0.05). N = 4–6 mice per group
Article Snippet: Levels of CTX-1 and P1NP were measured using the Serum Crosslaps (CTX-1) ELISA (Immunodiagnostic Systems #AC-02F1) and Rat/Mouse P1NP ELISA (Immunodiagnostic Systems #AC-33F1) according to the manufacturer’s protocols.
Techniques: Transformation Assay, Expressing, Concentration Assay, Activity Assay, Standard Deviation